Related Experiment Video
Updated: Dec 21, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Responses of microbial structures, functions, metabolic pathways and community interactions to different C/N ratios
Hongwei Sun1, Wenyan Shi2, Chenjian Cai2
1School of Environmental and Material Engineering, Yantai University, Yantai, Shandong, 264005, China.
Abstract:
The responses of microbial structures, functional profiles and metabolic pathways during nitrification to four C/N ratios (0, 5, 10 and 15) were investigated in four parallel SBRs denoted as S0, S5, S10, S15. Results indicated that microbial diversities were affected by C/N ratios, while the same dominant taxa were observed, mainly including Proteobacteria, Betaproteobacteria, Rhodocyclales, Rhodocyclaceae, Zoogloea. The unique biomarkers were identified in each sludge sample through LEfSe analysis. Functional genera/enzymes responsible for removing organics and nitrogen coexisted in four SBRs at different abundances, except for that ammonia oxidizing bacteria (AOB) Nitrosomonas (0.33%-0.66%) and ammonia monooxygenase (amo) (9.4 × 10-7-2.8 × 10-6) were only detected in S0. Moreover, PICRUSt analysis indicated similar overall patterns of metabolic pathways in four sludge samples. The network analysis revealed that total nitrogen removal positively correlated with hcp (Spearman's ρ of 0.853), and ammonia oxidizing rate was associated with amo (Spearman's ρ of 0.096).
More Related Videos
12:47Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
08:05Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
Related Concept Videos
Metabolism of Chemolithotrophs
Inorganic Nitrogen Assimilation
Microbial Nutrition
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
Carbon-dioxide Fixation
Environmental Applications of Microorganisms