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Updated: Dec 21, 2025
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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
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Chatterboxes: the structural and functional diversity of cyclins.
Natalie J Tatum1, Jane A Endicott1
1Cancer Research UK Newcastle Drug Discovery Unit, Newcastle Centre for Cancer, Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom.
Seminars in Cell & Developmental Biology
|May 17, 2020
Summary
This review surveys human cyclin structures, focusing on their roles in cell processes and regulation via degradation. It highlights the cyclin box domain
Area of Science:
- Molecular and Structural Biology
- Cellular Biology
- Biochemistry
Background:
- Cyclins are a diverse protein family with a conserved cyclin box domain, crucial for cell cycle regulation.
- Structural characterization of cyclins is essential for understanding their diverse cellular functions.
- Only a fraction of cyclin-dependent kinase (CDK)-activating cyclins have been structurally elucidated.
Purpose of the Study:
- To review available structural data of human cyclins.
- To correlate structural features with cellular roles and regulatory mechanisms.
- To explore emerging cyclin functions beyond CDK activation.
Main Methods:
- Survey of existing X-ray crystallography and cryo-electron microscopy structural data for human cyclins.
- Analysis of molecular features and their implications in cellular processes.
- Review of literature on cyclin regulation, degradation, and non-CDK roles.
Main Results:
- Detailed examination of molecular features of structurally characterized human cyclins.
- Emphasis on the regulation of cyclin activity through degradation motif recognition and ubiquitination.
- Identification of cyclin box domains as versatile scaffolds for protein-protein interactions.
Conclusions:
- Structural insights are key to understanding cyclin functions and regulation.
- Cyclin activity is precisely controlled by degradation pathways.
- The cyclin box domain serves as a fundamental platform for diverse protein interactions, extending beyond kinase activation.
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