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Updated: Dec 21, 2025
Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres
Published on: June 1, 2012
Quantum dots-βcyclodextrin-histidine labeled human adipose stem cells-laden chitosan hydrogel for bone tissue
Vahid Jahed1, Ebrahim Vasheghani-Farahani1, Fatemeh Bagheri2
1Biomedical Engineering Division, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran.
Abstract:
Mesenchymal stem cells with differentiation ability to diverse cells play a crucial role in tissue engineering. Tracking the fate of these cells during the regeneration of tissue helps to obtain more information about their function. In this study, histidine conjugated β-cyclodextrin as a cell-penetrating carrier with drug loading ability was attached to QDs nanoparticle (QD-βCD-His) for stem cell labeling. Traceability of QD-βCD-His labeled human adipose stem cells (hASCs) was monitored in 2D cell culture and 3D temperature-sensitive chitosan hydrogel scaffold. Dexamethasone (Dex) as an osteoinductive drug was loaded into QD-βCD-His nano-carrier (QD-βCD-His@Dex) to induce bone differentiation of labeled cells. Overall results indicated that QD-βCD-His@Dex is a promising dual-purpose nano-carrier for stem cell labeling with osteoinductive potential in cell therapy as well as tissue engineering scaffolds.
