Related Experiment Video
Updated: Dec 21, 2025

10:44
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
Published on: October 21, 2016
31.4K
NBD-based fluorescent probes for separate detection of cysteine and biothiols via different reactivities
Haojie Huang1, Xiuru Ji2, Yaqing Jiang1
1State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Technology, Beijing 100029, China. zhujq@mail.buct.edu.cn.
Organic & Biomolecular Chemistry
|May 19, 2020
Abstract:
NBD-based fluorescent probes that can separately detect cysteine and biothiols via different reactivities have been rationally designed and synthesized. The probes can be applied to kinetically distinguish cysteine from other biothiols at 30 min, and to detect all biothiols at 3 h in living cells.

