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Preparing Activated Platelet-Rich Plasma for Culturing Human Adipose-Derived Stem Cells
Fangyuan Lai1, Natsuko Kakudo2, Naoki Morimoto3
1Department of Plastic and Reconstructive Surgery, Kansai Medical University; Department of Plastic Surgery, Zhejiang Provincial People's Hospital.
Journal of Visualized Experiments : Jove
|May 19, 2020
Summary
Activated platelet-rich plasma (PRP) significantly boosts human adipose-derived stem cell (hASC) proliferation. This method offers a reproducible way to prepare concentrated PRP for regenerative medicine applications.
Area of Science:
- Regenerative Medicine
- Cell Biology
- Biotechnology
Background:
- Platelet-rich plasma (PRP) contains growth factors that stimulate cell proliferation.
- PRP has potential applications in regenerative medicine due to its mitogenic properties.
- Standard PRP preparation methods may vary in platelet concentration and efficacy.
Purpose of the Study:
- To develop and validate a double-spin method for preparing activated PRP.
- To quantify the platelet concentration in the prepared PRP.
- To evaluate the proliferation-stimulating effect of activated PRP on human adipose-derived stem cells (hASCs).
Main Methods:
- Whole blood was processed using a double-spin centrifugation technique to isolate PRP.
- Autologous thrombin was used to activate the prepared PRP.
- Platelet counts in PRP and whole blood plasma were measured.
- The effect of activated PRP on hASC proliferation was assessed through cell culture experiments.
Main Results:
- The double-spin method yielded PRP with a platelet concentration 11.5 times higher than whole blood plasma.
- Activated PRP significantly enhanced the proliferation of hASCs.
- A concentration of 1% activated PRP markedly promoted hASC proliferation in vitro.
Conclusions:
- The described double-spin method reliably produces PRP with a high platelet yield.
- Activated PRP prepared by this method demonstrates a potent proliferation-stimulating effect on hASCs.
- This optimized PRP preparation technique holds promise for advancing regenerative medicine strategies.
