Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A "three-in-one" nose-to-brain delivery strategy: intranasal vancomycin spray achieves simultaneous clearance of pneumococcal colonization, bacteremia, and meningitis.

International journal of pharmaceutics·2026
Same author

Cuproptosis-Related Genes in Immune Infiltration and Diagnosis in Hepatitis B Virus-Related Acute Liver Failure.

Exploration (Beijing, China)·2026
Same author

Microneedle technology integrated with diverse therapeutic modalities for hair regrowth in alopecia.

Acta pharmaceutica Sinica. B·2026
Same author

Aggregation-Induced Emission (AIE) Probe-Labeled Nanotheranostics: A Mini-Review.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Toward an AI Era: Application of Artificial Intelligence in Inclusion Complex Screening.

Pharmaceutics·2026
Same author

Light-driven thermotropic hydrogel with nanomodulator enables lipid intervention and cavity delivery for postsurgical tumor therapy.

Cell reports. Medicine·2026

Related Experiment Video

Updated: Dec 21, 2025

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

14.8K

Dissolving Microneedle Arrays with Optimized Needle Geometry for Transcutaneous Immunization.

Yingying Li1, Xia Hu1, Zhiyong Dong1

  • 1Faculty of Pharmacy, Bengbu Medical College, Bengbu, China.

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
|May 19, 2020
PubMed
Summary

Dissolving microneedle arrays (DMNA) effectively deliver ovalbumin (OVA) for transcutaneous immunization. Cone-shaped DMNA enhance immune responses compared to traditional needles, paving the way for vaccine development.

Keywords:
Different needle-geometriesDissolving microneedle array (DMNA)Immunoglobulin G (IgG)Ovalbumin (OVA)SDS-PAGETranscutaneous immunizationTwo-step molding

More Related Videos

Polymeric Microneedle Array Fabrication by Photolithography
08:15

Polymeric Microneedle Array Fabrication by Photolithography

Published on: November 17, 2015

12.7K
Author Spotlight: Innovative Microneedle-Based Strategies for Enhanced Exosome Delivery and Stability
07:41

Author Spotlight: Innovative Microneedle-Based Strategies for Enhanced Exosome Delivery and Stability

Published on: July 12, 2024

3.2K

Related Experiment Videos

Last Updated: Dec 21, 2025

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
08:19

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Published on: June 1, 2012

14.8K
Polymeric Microneedle Array Fabrication by Photolithography
08:15

Polymeric Microneedle Array Fabrication by Photolithography

Published on: November 17, 2015

12.7K
Author Spotlight: Innovative Microneedle-Based Strategies for Enhanced Exosome Delivery and Stability
07:41

Author Spotlight: Innovative Microneedle-Based Strategies for Enhanced Exosome Delivery and Stability

Published on: July 12, 2024

3.2K

Area of Science:

  • Biotechnology
  • Immunology
  • Materials Science

Background:

  • Transcutaneous immunization offers a needle-free alternative for vaccine delivery.
  • Dissolving microneedle arrays (DMNA) are emerging as promising carriers for transdermal drug delivery.
  • Optimizing DMNA geometry is crucial for effective antigen delivery and immune response.

Purpose of the Study:

  • To investigate the feasibility of using DMNA with varying needle geometries for transcutaneous immunization.
  • To evaluate DMNA as drug carriers for ovalbumin (OVA) preparations.
  • To compare the immunogenicity of DMNA-based delivery with conventional hypodermic injections.

Main Methods:

  • Fabrication of DMNA with different needle geometries using a two-step molding process.
  • Characterization of DMNA, including drug loading (~100 μg OVA) and stability assessment (SDS-PAGE).
  • In vivo comparison of immune responses (IgG production) induced by DMNA and hypodermic injections.

Main Results:

  • DMNA successfully loaded with OVA, demonstrating stability during preparation.
  • DMNA with different needle geometries were fabricated and characterized.
  • OVA-loaded DMNA induced significantly stronger immune responses than hypodermic needle injections.

Conclusions:

  • Microneedle morphology critically influences DMNA's mechanical properties, insertion, dissolution, and subsequent immune response.
  • Cone-shaped DMNA are optimal for transcutaneous immunization.
  • DMNA represent a viable platform for vaccine development, offering enhanced immunogenicity via transcutaneous immunization.