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Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing
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Surface Enhanced Raman Spectroscopy Based Biosensor with a Microneedle Array for Minimally Invasive In Vivo Glucose

Jian Ju1,2, Chao-Mao Hsieh1, Yao Tian1,3

  • 1School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore.

ACS Sensors
|May 20, 2020
PubMed
Summary
This summary is machine-generated.

Diabetic patients can now have painless glucose monitoring using a novel microneedle sensor. This surface-enhanced Raman spectroscopy (SERS) biosensor offers accurate, in-situ detection in interstitial fluid, reducing fingerstick tests.

Keywords:
glucose sensingin vivo biosensormicroneedle arrayminimally invasive intradermal measurementssurface enhanced Raman scattering

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Materials Science

Background:

  • Diabetes management requires frequent blood glucose monitoring, often involving painful fingerstick tests.
  • Current methods pose risks of cross-contamination and inconvenience for patients.
  • There is a need for minimally invasive, accurate, and reliable glucose monitoring technologies.

Purpose of the Study:

  • To develop a novel surface-enhanced Raman spectroscopy (SERS) sensor for in-situ intradermal glucose detection.
  • To evaluate the efficacy of a poly(methyl methacrylate) microneedle (PMMA MN) array for painless glucose monitoring.
  • To assess the accuracy and safety of the developed biosensor in a diabetic mouse model.

Main Methods:

  • Fabrication of a silver-coated poly(methyl methacrylate) microneedle (PMMA MN) array functionalized with 1-decanethiol (1-DT).
  • Calibration of the sensor in skin phantoms within the 0-20 mM glucose range.
  • In vivo testing for interstitial fluid (ISF) glucose quantification in streptozotocin (STZ)-induced diabetic mice.
  • Analysis of sensor performance using Clarke error grid and assessment of skin recovery.

Main Results:

  • The functional PMMA MN array successfully measured glucose in ISF within minutes.
  • The sensor maintained structural integrity without swelling during measurements.
  • Clarke error grid analysis showed 93% of data points in clinically acceptable zones A and B.
  • The microneedle array demonstrated minimal invasiveness with rapid skin recovery (within 10 min).

Conclusions:

  • The developed polymeric microneedle array-based SERS biosensor enables painless, in-situ glucose monitoring.
  • This technology shows significant potential for improving diabetes management by replacing painful fingerstick tests.
  • Further validation could lead to a practical tool for diabetic patients' daily glucose monitoring.