Related Experiment Video
Updated: Dec 21, 2025

Concurrent Quantification of Cellular and Extracellular Components of Biofilms
Published on: December 10, 2013
Morphology and Ultrastructure of Group A Streptococcus Biofilms
Tatyana A Danilova1, T A Smirnova2, G A Danilina2
1N.F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia. dansen@inbox.ru.
Abstract:
Light and electron microscopy enables researchers to study the ultrastructure of GAS biofilms formed on abiotic surfaces. Chains of streptococci surrounded by a bluish film are seen under a light microscope after alcian blue staining of preparations grown on coverslips. The extracellular matrix (indicator of biofilm maturity) becomes visible on ultrathin sections in transmission electron microscopy after additional staining with alcian blue; filamentous structures, characteristic of biofilm, are observed in intercellular spaces. The data obtained by scanning electron microscopy also demonstrate the presence of biofilm.
More Related Videos
09:45A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy
Published on: January 25, 2017
11:09An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
Published on: January 25, 2011
Related Concept Videos
Biofilms
Bacterial Phylum Actinobacteria
Surface Appendages of Archaea
Microbial Morphologies