The age-dependent effect of pre-pubertal castration on anxiety-like behaviour in male rats

Adolescence is considered to be a critical period of sex hormone action (re)organising the brain and determining the behavioural phenotype. Such organisational effects in the brain might be the cause of sex differences in some behavioural features. In this experiment, we aimed to examine the role of pubertal sex hormones in development of anxiety in male rats. Male rats underwent gonadectomy prior to puberty onset, and were tested for explorative and anxiety-like behaviour in adolescence as well as in young adulthood. In adolescence, but not in adulthood, gonadectomised rats spend by 50% more time (p < .05) in the centre zone of the open-field than sham-operated counterparts. Young adult gonadectomised rats showed approximately 1.5-fold greater exploratory activity, in both open field (p < .001) and elevated plus maze (p < .01), in comparison with young adult control rats. Our results indicate that pre-pubertal castration may have test-specific anxiolytic effect in adolescent male rats, and it may attenuate the decline in explorative behaviour in young adult males. These differences in short- and long-term effects of gonadectomy could explain some contradictory results of previous studies on the role of testosterone in anxiety-like behaviour of male rodents. Thus, the age-specific consequences of pre-pubertal hormone deprivation should be considered.