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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Related Experiment Video

Updated: Dec 20, 2025

Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus
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Osteochondral Regeneration Using Adipose Tissue-Derived Mesenchymal Stem Cells.

Daiki Murata1, Ryota Fujimoto1,2, Koichi Nakayama1

  • 1Center for Regenerative Medicine Research, Faculty of Medicine, Saga University, Honjo-machi, Saga 840-8502, Japan.

International Journal of Molecular Sciences
|May 23, 2020
PubMed
Summary

Adipose tissue-derived mesenchymal stem cells (AT-MSCs) show promise for regenerating joint tissues in osteoarthritis. Scaffold-free 3D constructs using AT-MSC spheroids offer a potential solution for osteochondral regeneration.

Keywords:
Kenzan methodadipose tissue-derived mesenchymal stem cellscell constructmold methodosteoarthritisosteochondral regenerationscaffold-freespheroid

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Clinical Protocol of Producing Adipose Tissue-Derived Stromal Vascular Fraction for Potential Cartilage Regeneration
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Area of Science:

  • Regenerative Medicine
  • Biomaterials Engineering
  • Orthopedics

Background:

  • Osteoarthritis (OA) is a debilitating joint disease causing locomotor deficiency and reduced quality of life.
  • Current surgical strategies focus on reconstructing articular cartilage and subchondral bone for OA.
  • Adipose tissue-derived mesenchymal stem cells (AT-MSCs) possess pluripotency and self-proliferation, making them candidates for osteochondral regeneration.

Purpose of the Study:

  • To review studies on adipose tissue-derived mesenchymal stem cells (AT-MSCs) for osteochondral regeneration in osteoarthritis.
  • To explore the use of AT-MSC spheroids and three-dimensional (3D) constructs in cartilage and subchondral bone repair.
  • To discuss the potential of scaffold-free 3D constructs for osteochondral regeneration.

Main Methods:

  • Review of existing literature on AT-MSC applications in osteochondral regeneration.
  • Analysis of studies utilizing AT-MSC spheroids and 3D constructs (scaffold and scaffold-free).
  • Examination of fabrication methods like "mold" and "Kenzan" for 3D constructs.

Main Results:

  • AT-MSCs have demonstrated potential in regenerating articular cartilage.
  • Studies show AT-MSC applications extend to regenerating both cartilage and subchondral bone (osteochondral regeneration).
  • Scaffold-free 3D constructs using AT-MSC spheroids show promise for osteochondral repair.

Conclusions:

  • AT-MSCs, particularly in spheroid and scaffold-free 3D forms, represent a promising avenue for osteochondral regeneration.
  • Fabrication methods like "mold" and "Kenzan" are key to creating effective AT-MSC constructs.
  • Further research into scaffold-free 3D constructs holds significant prospects for treating osteoarthritis.