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Rapid isolation of thymosin beta 4 from thymosin fraction 5 by preparative high-performance liquid chromatography
M Badamchian1, M P Strickler, M J Stone
1Department of Biochemistry, George Washington University, School of Medicine, Washington, DC 20037.
Abstract:
We have developed a rapid, efficient, and reproducible two-step method for the purification of thymosin beta 4 (T beta 4) from thymosin fraction 5 (TF5). This purification is based on the use of high-performance preparative/semi-preparative and analytical reversed-phase (Delta-Pak C18) chromatographic columns. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and amino acid compositional analysis have shown that natural, purified T beta 4 is identical to synthetic T beta 4. This procedure can be used to isolate other biologically active peptides from TF5 in sufficient quantity for characterization.