Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Determination01:51

Determination

20.5K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
20.5K
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

3.7K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
3.7K
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

3.8K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
3.8K
Cell Migration01:19

Cell Migration

6.2K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.2K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

3.4K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.4K
Gastrulation01:56

Gastrulation

65.0K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
65.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blind But Alive - Congenital Loss of atoh7 Disrupts the Visual System of Adult Zebrafish.

Investigative ophthalmology & visual science·2024
Same author

Inflammation is a critical factor for successful regeneration of the adult zebrafish retina in response to diffuse light lesion.

Frontiers in cell and developmental biology·2024
Same author

Dose and dose rate dependence of the tissue sparing effect at ultra-high dose rate studied for proton and electron beams using the zebrafish embryo model.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2024
Same author

The DRESDEN PLATFORM is a research hub for ultra-high dose rate radiobiology.

Scientific reports·2023
Same author

Single-cell RNA sequencing unravels the transcriptional network underlying zebrafish retina regeneration.

eLife·2023
Same author

Real-time monitoring of an endogenous Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation.

Development (Cambridge, England)·2023
Same journal

Dissecting planar and vertical organiser signals in early chick neural development.

Development (Cambridge, England)·2026
Same journal

Real-time transcriptomic profiling of hPSC-derived cartilage during development identifies a key role for the extracellular matrix in homeostasis and protection.

Development (Cambridge, England)·2026
Same journal

In preprints - housekeeping the housekeeping genes.

Development (Cambridge, England)·2026
Same journal

In preprints - light, cluster, friction: a cell dance on the gastrulation stage.

Development (Cambridge, England)·2026
Same journal

PBX-dependent and -independent Hox programs establish and maintain motor neuron terminal identity.

Development (Cambridge, England)·2026
Same journal

NUDT21 regulates 3'UTR dynamics in epididymal principal cells to preserve sperm integrity.

Development (Cambridge, England)·2026
See all related articles

Related Experiment Video

Updated: Dec 20, 2025

Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro
07:33

Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

Published on: January 31, 2012

18.4K

Cell-fate plasticity, adhesion and cell sorting complementarily establish a sharp midbrain-hindbrain boundary.

Gokul Kesavan1, Anja Machate1, Stefan Hans1

  • 1Center for Regenerative Therapies TU Dresden (CRTD), Technische Universität Dresden, Fetscherstr. 105, 01307 Dresden, Germany.

Development (Cambridge, England)
|May 23, 2020
PubMed
Summary
This summary is machine-generated.

Cell fate boundaries in embryonic development are crucial. This study reveals how the midbrain-hindbrain boundary (MHB) uses cell sorting via differential adhesion to maintain distinct cell populations.

Keywords:
Cell adhesionCell sortingEphrin signalingMidbrain-hindbrain boundary (MHB)Zebrafish

More Related Videos

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 12, 2014

12.2K
Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
10:57

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

Published on: March 18, 2014

11.6K

Related Experiment Videos

Last Updated: Dec 20, 2025

Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro
07:33

Organotypic Slice Cultures of Embryonic Ventral Midbrain: A System to Study Dopaminergic Neuronal Development in vitro

Published on: January 31, 2012

18.4K
Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis
09:52

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Published on: March 12, 2014

12.2K
Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
10:57

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

Published on: March 18, 2014

11.6K

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Neuroscience

Background:

  • Sharp boundaries between cell groups are vital for embryonic development, compartmentalizing cells and organizing development.
  • The midbrain-hindbrain boundary (MHB) acts as an organizer and a lineage restriction boundary, preventing cell fate intermingling, but its mechanisms are unclear.

Purpose of the Study:

  • To elucidate the mechanisms underlying lineage restriction at the midbrain-hindbrain boundary (MHB).
  • To provide mechanistic details on how sharp gene expression boundaries form in the developing MHB.

Main Methods:

  • Novel fluorescent knock-in reporters
  • Live imaging
  • Cre/lox-mediated lineage tracing
  • Atomic force microscopy-based cell adhesion assays
  • Mutant analysis

Main Results:

  • Lineage restriction at the MHB is established by the end of gastrulation.
  • Sharp gene expression boundaries form through cell-fate plasticity and cell sorting.
  • Cell sorting at the MHB involves differential adhesion mediated by N-cadherin and Eph-ephrin signaling.

Conclusions:

  • The study provides mechanistic insights into lineage restriction at the MHB.
  • Differential cell adhesion is a key mechanism for maintaining cell segregation at the MHB.
  • This research clarifies how developmental boundaries are formed and maintained.