The long non-coding RNA LINC00473 contributes to cell proliferation via JAK-STAT3 signaling pathway by regulating

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Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
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Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Published on: November 26, 2015

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Zengshu Xing1, Sailian Li2, Zhenxiang Liu1

  • 1Department of Urology, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, No.43, Renmin Road, Meilan District, Haikou, 570208, Hainan Province, P.R. China.

Bioscience Reports
|May 23, 2020
PubMed

Insights

Long non-coding RNA LINC00473 promotes prostate cancer cell proliferation by regulating the miR-195-5p/SEPT2 axis and JAK-STAT3 pathway. Inhibiting LINC00473 offers a potential therapeutic strategy for prostate cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Prostate cancer poses a significant health threat due to high incidence and metastasis.
  • Understanding the molecular mechanisms of prostate cancer is crucial for effective treatment.
  • LINC00473, a long non-coding RNA, has known roles in various cancers, but its function in prostate cancer was unclear.

Purpose of the Study:

  • To investigate the role of LINC00473 in prostate cancer.
  • To elucidate the molecular mechanism by which LINC00473 influences prostate cancer progression.
  • To explore LINC00473 as a potential therapeutic target for prostate cancer.

Main Methods:

  • Quantitative real-time PCR to measure LINC00473 expression.
  • Cell proliferation assays to assess the impact of LINC00473.
  • Western blotting to detect protein expression in the JAK-STAT3 pathway.
  • RNA immunoprecipitation and dual-luciferase reporter assays to confirm molecular interactions.

Main Results:

  • Prostate cancer cells exhibited high expression of LINC00473.
  • LINC00473 inhibition suppressed cell proliferation and JAK-STAT3 signaling.
  • LINC00473 negatively regulated miR-195-5p, which in turn regulated SEPT2.
  • SEPT2 overexpression rescued the inhibitory effects of LINC00473 knockdown.

Conclusions:

  • LINC00473 promotes prostate cancer cell proliferation through the JAK-STAT3 signaling pathway.
  • The LINC00473/miR-195-5p/SEPT2 axis is a key regulatory mechanism in prostate cancer.
  • Targeting LINC00473 presents a novel therapeutic avenue for prostate cancer treatment.
Keywords:
JAK-STAT3 signaling pathwayLINC00473SEPT2miR-195-5pprostate cancer

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