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External Quality Assessment Program for Next-Generation Sequencing-Based HIV Drug Resistance Testing: Logistical
Hezhao Ji1,2, Neil Parkin3, Feng Gao4
1National HIV and Retrovirology Laboratories at JC Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada.
Viruses
|May 24, 2020
Summary
External quality assessment (EQA) is crucial for next-generation sequencing (NGS) HIV drug resistance (HIVDR) genotyping assays. Discussions at a symposium identified challenges and recommendations for developing an EQA program for NGS HIVDR.
Area of Science:
- Clinical diagnostics
- Molecular biology
- Public health
Background:
- Next-generation sequencing (NGS) is emerging as the standard for HIV drug resistance (HIVDR) genotyping.
- External quality assessment (EQA) is a critical, yet often missing, component for clinical NGS HIVDR assays.
- Ensuring assay consistency and laboratory competency is vital for reliable HIVDR genotyping.
Purpose of the Study:
- To discuss and identify challenges in developing EQA strategies for NGS HIVDR assays.
- To summarize expert opinions and recommendations for establishing an inaugural EQA program.
- To address the need for EQA to support the clinical implementation of NGS HIVDR testing.
Main Methods:
- A multidisciplinary panel of experts convened at the 2nd International Symposium on NGS HIVDR.
- Themed discussions focused on EQA strategies for NGS HIVDR assays.
- Expert opinions and identified logistical challenges were documented.
Main Results:
- Logistical challenges in implementing an EQA program for NGS HIVDR were identified.
- Key recommendations and expert opinions were gathered to guide EQA development.
- The symposium highlighted the necessity of EQA for clinical NGS HIVDR adoption.
Conclusions:
- The development of an EQA program is essential for the widespread clinical adoption of NGS HIVDR testing.
- Expert consensus provides a foundation for creating a robust EQA framework.
- Addressing identified challenges will facilitate the establishment of reliable EQA for NGS HIVDR.
