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Updated: Dec 20, 2025
Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain Donors
Published on: January 21, 2017
Generation of four postmortem dura-derived iPS cell lines from four control individuals with genotypic and
Tomoyo Sawada1, Kynon J M Benjamin2, Anna C Brandtjen3
1Lieber Institute for Brain Development, Baltimore, Maryland, USA; Department of Neurology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
Abstract:
In this study, we established induced pluripotent stem (iPS) cell lines from postmortem dura-derived fibroblasts of four control individuals with low polygenic risk score for psychiatric disorders including schizophrenia and bipolar disorder. The fibroblasts were reprogrammed into iPS cells using episomal vectors carrying OCT3/4, SOX2, KLF4, L-Myc, LIN28 and shRNA-p53. All iPS cell lines showed the same genotype with parental postmortem brain tissues, expressed pluripotency markers, and exhibited the differentiation potency into three embryonic germ layers.
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