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Updated: Dec 20, 2025
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Published on: March 31, 2021
Evaluation of a high-speed but low-throughput RT-qPCR system for detection of SARS-CoV-2
J Sakai1, N Tarumoto1, Y Orihara2
1Department of Infectious Disease and Infection Control, Saitama Medical University, Saitama, Japan; Centre of Clinical Infectious Disease and Research, Saitama Medical University, Saitama, Japan.
Abstract:
With the emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), a high-speed and convenient detection technology should be at the forefront of medical care worldwide. This study evaluated the usefulness of GeneSoC, a compact, high-speed reciprocal flow quantitative reverse transcription polymerase chain reaction system, for the detection of SARS-CoV-2. The results support the use of this system for the rapid identification of SARS-CoV-2. This approach can contribute to the strategic selection of initial management strategies for patients with COVID-19.
