Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Developments in processing image sequences for elemental mapping.

N Bonnet1, C Colliex, C Mory

  • 1Unité de Service de Microscopie Electronique Analytique et Quantitative, Université Paris Sud, Orsay, France.

Scanning Microscopy. Supplement
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intake of neutral core human milk oligosaccharides (HMOs) during early life improves long-term bone quality.

The Journal of nutritional biochemistry·2025
Same author

Impact of therapeutic drug monitoring of antibiotics in the management of infective endocarditis.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2022
Same author

Inoculum effect of Enterobacterales co-expressing OXA-48 and CTX-M on the susceptibility to ceftazidime/avibactam and meropenem.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2022
Same author

Higher serum levels of a cathepsin K-generated periostin fragment are associated with fractures in postmenopausal women with primary hyperparathyroidism: a pilot study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2021
Same author

Lymphomatoid papulosis types D and E: a multicentre series of the French Cutaneous Lymphomas Study Group.

Clinical and experimental dermatology·2021
Same author

Two-year follow-up of 196 interstitial lung disease patients after ICU stay.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2021
Same journal

Proceedings of the 14th Pfefferkorn Conference. Belleville, Illinois, USA. August 6-11, 1995.

Scanning microscopy. Supplement·1996
Same journal

Advanced instrumentation and methodology related to cryoultramicrotomy: a review.

Scanning microscopy. Supplement·1996
Same journal

Aspects of cryofixation and cryosectioning for the observation of bulk biological samples in the hydrated state by cryoelectron microscopy.

Scanning microscopy. Supplement·1996
Same journal

In vitro systems and cultured cells as specimens for X-ray microanalysis.

Scanning microscopy. Supplement·1996
Same journal

X-ray microscopy: preparations for studies of frozen hydrated specimens.

Scanning microscopy. Supplement·1996
Same journal

Specimen preparation of the human cerebellar cortex for scanning electron microscopy using a t-butyl alcohol freeze-drying device.

Scanning microscopy. Supplement·1996
See all related articles

Accurate elemental mapping using electron energy loss spectroscopy (EELS) requires precise background estimation. This study evaluates various methods to minimize extrapolation errors, improving quantitative elemental mapping and single-atom detection.

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Microscopy

Background:

  • Elemental mapping visualizes chemical species distribution in specimens using EELS core signals from energy-filtered images.
  • A key challenge in EELS elemental mapping is background extrapolation error, leading to false positives or negatives.
  • Accurate background estimation is crucial for reliable elemental quantification and sensitivity.

Purpose of the Study:

  • To assess the validity of different background modeling and fitting procedures for EELS elemental mapping.
  • To improve the reliability and accuracy of quantitative elemental mapping.
  • To advance the identification of single atoms in materials.

Main Methods:

  • Evaluation of diverse mathematical background models.

Related Experiment Videos

  • Comparison of one-parameter and two-parameter fitting procedures.
  • Analysis of various fitting methods, including quasi-local parameter estimation.

    • Main Results:

    • Statistical validity of different background estimation techniques was tested on real EELS datasets.
    • Performance was assessed using specimens like Co/CeO2 catalysts, ferritin molecules, and U/Tb staining clusters.
    • Progress towards quantitative elemental mapping with defined confidence levels was demonstrated.

    Conclusions:

    • The study provides insights into optimizing background subtraction for EELS elemental mapping.
    • Improved methods enhance the accuracy of chemical species distribution analysis.
    • This work contributes to the goal of identifying individual atoms through advanced microscopy techniques.