Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K
RNA-seq03:21

RNA-seq

11.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adapting a Youth Sexual Violence Prevention Logic Model and Evaluation to Asian American and Pacific Islander Youth in Iowa: a Case Study.

Prevention science : the official journal of the Society for Prevention Research·2025
Same author

[Clinical features and prognosis associated risk factors analysis of non-eosinophilic esophagitis eosinophilic gastrointestinal diseases in children].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2025
Same author

[DICER1-mutant primary intracranial sarcoma: analysis of five cases].

Zhonghua bing li xue za zhi = Chinese journal of pathology·2025
Same author

[Reinforcement of personalized management of type 2 diabetes mellitus].

Zhonghua yi xue za zhi·2025
Same author

[Progress in the therapy of corneal neovascularization].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2025
Same author

[Interpretation of the "Technical Guidelines for Disinfection in Epidemic Prevention and Control of Large-Scale Events"].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025

Related Experiment Video

Updated: Dec 20, 2025

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
07:29

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

6.6K

[Transcriptome profiling of differentiated lenses through RNA sequencing].

L F Zhang1, Z W Qin1, B Lu1

  • 1Eye Center, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China.

[Zhonghua Yan Ke Za Zhi] Chinese Journal of Ophthalmology
|May 26, 2020
PubMed
Summary

The lens transcriptome reveals overlapping expression of mRNA, long non-coding RNA, and circular RNA during differentiation. Key lens-specific genes like crystallin and gap junction proteins show distinct expression patterns.

Keywords:
Gene expression profilingHigh-throughput nucleotide sequencingLens, crystallineRNA, circularRNA, long noncodingRNA, messenger

More Related Videos

RNA Isolation from Mouse Ocular Lens Epithelium and Fiber Cell Bulk Masses
06:07

RNA Isolation from Mouse Ocular Lens Epithelium and Fiber Cell Bulk Masses

Published on: October 10, 2025

357
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

4.0K

Related Experiment Videos

Last Updated: Dec 20, 2025

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
07:29

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

Published on: May 16, 2020

6.6K
RNA Isolation from Mouse Ocular Lens Epithelium and Fiber Cell Bulk Masses
06:07

RNA Isolation from Mouse Ocular Lens Epithelium and Fiber Cell Bulk Masses

Published on: October 10, 2025

357
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

4.0K

Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Genomics

Background:

  • The differentiated lens transcriptome, including mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA), is not fully understood.
  • Investigating the transcriptional landscape is crucial for understanding lens development and function.

Purpose of the Study:

  • To comprehensively analyze the transcriptional landscape of the differentiated lens.
  • To identify overlapping and differentially expressed RNAs at various differentiation stages.
  • To examine the expression patterns of lens-specific genes.

Main Methods:

  • RNA sequencing (Illumina HiSeq 2500) of differentiated lenses at 16, 23, and 25 weeks.
  • Bioinformatic analysis to screen top expressed and differentially expressed mRNAs, lncRNAs, and circRNAs.
  • Venn diagram analysis for overlapping gene expression and real-time PCR for verification of lens-specific gene expression tendencies.

Main Results:

  • High numbers of mapped reads were obtained across differentiation stages.
  • Significant overlap in the expression of top mRNAs (740/1000), lncRNAs (170/300), and circRNAs (69/100) was observed.
  • Lens-specific genes, including crystallins (CRY), were upregulated, while gap junction genes (GJ A3, GJA8) were downregulated with lens differentiation.

Conclusions:

  • A substantial proportion of highly expressed mRNAs, lncRNAs, and circRNAs show overlapping expression across lens differentiation stages.
  • The differentiated lens exhibits high expression of specific protein genes, such as crystallins and gap junctions.
  • This study provides a detailed profile of the lens transcriptome, highlighting key regulatory elements during differentiation.