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Updated: Dec 20, 2025

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
Published on: November 22, 2019
jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths
Manuel Alexander Mohr1,2,3, Daniel Bushey1, Abhi Aggarwal1,4
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Abstract:
Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.
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