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Updated: Dec 20, 2025
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Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules
Published on: August 16, 2011
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Identification of Transposable Elements in Schistosoma mansoni
Gisele S Philippsen1, Ricardo DeMarco2
1Universidade Federal do Paraná, Jandaia do Sul, Paraná, Brazil. gistrieder@ufpr.br.
Methods in Molecular Biology (Clifton, N.J.)
|May 27, 2020
Summary
We present protocols for identifying, curating, and mapping transposable elements (TEs) in the Schistosoma mansoni genome. These methods aid in understanding TE influence on genome architecture and evolution.
Area of Science:
- Genomics
- Molecular Biology
- Evolutionary Biology
Background:
- Transposable elements (TEs) constitute a substantial fraction of eukaryotic genomes.
- TEs significantly influence genome dynamics, evolution, and architecture.
- Understanding TE distribution is crucial for genome analysis.
Purpose of the Study:
- To describe protocols for identifying and curating consensus transposable element (TE) sequences from Schistosoma mansoni.
- To outline methods for mapping these identified TEs within the S. mansoni genome.
- To provide a resource for researchers studying TEs in S. mansoni and other organisms.
Main Methods:
- Bioinformatic analysis for TE identification and consensus sequence generation.
- Sequence curation to ensure accuracy and representativeness.
- Genome mapping techniques to determine TE locations within S. mansoni.
Main Results:
- Established protocols for TE identification and curation in S. mansoni.
- Developed methods for mapping TEs to the S. mansoni genome.
- Generated a foundational dataset and methodology for TE analysis in this species.
Conclusions:
- The described protocols facilitate comprehensive analysis of transposable elements in Schistosoma mansoni.
- These methods contribute to a deeper understanding of TE roles in genome evolution.
- The protocols are adaptable for TE research in other eukaryotic genomes.
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