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![Radiosynthesis of 1-2-[18F]Fluoroethyl-L-Tryptophan using a One-pot, Two-step Protocol](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F63025.jpg&w=3840&q=50)
Radiosynthesis of 1-2-[18F]Fluoroethyl-L-Tryptophan using a One-pot, Two-step Protocol
Published on: September 21, 2021
Modular control of l-tryptophan isotopic substitution via an efficient biosynthetic cascade
Clayton M Thompson1, Allwin D McDonald1, Hanming Yang1
1Department of Chemistry, University of Wisconsin-Madison, 1101 University Ave, Madison, WI. 53706, USA. cavagnero@chem.wisc.edu arbuller@wisc.edu.
Abstract:
Isotopologs are powerful tools for investigating biological systems. We report a biosynthetic-cascade synthesis of Trp isotopologs starting from indole, glycine, and formaldehyde using the enzymes l-threonine aldolase and an engineered β-subunit of tryptophan synthase. This modular route to Trp isotopologs is simple and inexpensive, enabling facile access to these compounds.
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