Quantification of Galantamine in Sternbergia Species by High Performance Liquid Chromatography
Özlem Bahadır Acikara1, Betül Sever Yilmaz1, Dilhun Yazgan1
1Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey.
Objectives:
This study describes the qualitative and quantitative analysis of galantamine in Sternbergia species growing in Turkey.
Materials And Methods:
Galantamine was isolated from Sternbergia fischeriana bulbs and the structure of the compound elucidated by spectroscopic methods. The qualitative and quantitative analysis of galantamine was investigated in Sternbergia lutea subsp. lutea, S. lutea subsp. sicula, Sternbergia candida, S. fischeriana, and Sternbergia clusiana using a specially developed and validated high performance liquid chromatography (HPLC) method.
Results:
S. lutea subsp. sicula had the highest content of galantamine, i.e., 0.0165±0.0002 g/100 g. The limits of detection and quantification were 7.5 μg and 25 μg, respectively.
Conclusion:
Isolation of galantamine from S. fischeriana growing in Turkey is reported for the first time. An HPLC method was developed for identification and quantification of galantamine in Sternbergia species.
