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Related Experiment Video

Updated: Dec 20, 2025

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Published on: August 5, 2020

Imaging Single Bacterial Cells with Electro-optical Impedance Microscopy.

Fenni Zhang¹, Shaopeng Wang¹, Yunze Yang¹

¹Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, Arizona 85287-5801, United States.

|May 28, 2020

Summary

Electro-optical impedance microscopy (EIM) images single bacteria impedance with subcellular resolution. This label-free technique monitors bacterial viability and heterogeneous responses to antibiotics, offering a new tool for cell studies.

Keywords:

cell viability label-free detection optical imaging potential modulation single bacteria impedance

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Last Updated: Dec 20, 2025

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Area of Science:

Biophysics
Microscopy
Biosensing

Background:

Impedance measurements are crucial for biosensor applications like protein detection and cell studies.
Existing methods lack subcellular resolution for single bacteria impedance imaging.

Purpose of the Study:

To introduce electro-optical impedance microscopy (EIM) for high-resolution single bacteria impedance imaging.
To demonstrate EIM's capability in monitoring bacterial viability and antibiotic responses.

Main Methods:

Applied potential modulation to bacteria on an indium tin oxide slide.
Recorded transmitted microscopy images and performed Fast Fourier Transform analysis.
Obtained DC component for morphology and AC component for impedance mapping.

Main Results:

Achieved subcellular resolution mapping of cell impedance responses for the first time.
Monitored *Escherichia coli* viability under antibiotic treatment using low-frequency potential modulation.
Observed heterogeneous responses to membrane-targeting antibiotics at the single-cell level.

Conclusions:

EIM is a label-free, high-resolution technique for single bacteria impedance imaging.
EIM can reveal heterogeneous cellular responses to antibiotics.
EIM has potential for continuous mapping of impedance changes in single bacteria.