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Related Concept Videos

Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
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Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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Related Experiment Video

Updated: Jul 10, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

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Published on: February 14, 2022

Ultrarapid Kinetic Antimicrobial Susceptibility Testing from Blood Using Single-Cell Scattering Phenotypic Imaging.

Jiahao Xu1, Yunrui Zhang1, Yueqin Hong2

  • 1Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China.

ACS Sensors
|July 8, 2026
PubMed
Summary
This summary is machine-generated.

Rapid antimicrobial susceptibility testing (AST) for bloodstream infections (BSIs) is now possible within hours using a novel single-cell imaging platform. This technology accelerates diagnosis and guides targeted therapy, improving patient outcomes and antimicrobial stewardship.

Keywords:
bloodstream infectionkinetic growth analysislarge-field single-cell imagingscattering phenotypic imagingultrarapid antimicrobial susceptibility testing

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Published on: July 9, 2012

Area of Science:

  • Microbiology
  • Medical Diagnostics
  • Optical Imaging

Background:

  • Bloodstream infections (BSIs) require timely antimicrobial susceptibility testing (AST) for effective treatment.
  • Current AST methods, relying on blood culture, have a turnaround time of 48-72 hours, delaying critical therapy.
  • There is a need for faster AST methods to enable early, targeted treatment of BSIs.

Purpose of the Study:

  • To develop and validate an ultrarapid phenotypic antimicrobial susceptibility testing (AST) platform for bloodstream infections (BSIs).
  • To enable AST directly from complex blood samples within hours, bypassing conventional blood culture enrichment.
  • To provide a foundation for earlier precision therapy and improved antimicrobial stewardship.

Main Methods:

  • A kinetic, single-cell nanoscale scattering phenotypic imaging platform was developed for large-volume, mm³-scale observation with single-cell sensitivity.
  • Bacteria were visualized and enumerated as nanoscale optical scatters without isolation, labeling, or microfluidic trapping.
  • A growth-inhibition kinetic model analyzed single-cell population trajectories to determine minimum inhibitory concentrations and categorical susceptibility.

Main Results:

  • The platform determined AST results within 2-3 hours for high bacterial loads (≥10³ CFU mL⁻¹) and <8 hours for ultralow loads (∼2-10 CFU mL⁻¹).
  • Testing on clinical BSI samples showed 96% categorical agreement across 11 antibiotics in 100 tests.
  • Validation in whole blood demonstrated a broad operating range (2-10⁷ CFU mL⁻¹), high sensitivity, and resilience to matrix interference.

Conclusions:

  • This imaging-only, reagent-minimal workflow enables clinically actionable AST within hours.
  • The platform offers a practical solution for earlier precision therapy in critical infection management.
  • The technology significantly improves upon traditional AST turnaround times, enhancing antimicrobial stewardship.