Experimental Platform Using the Amphibian Xenopus laevis for Research in Fundamental and Medical Immunology

The amphibian Xenopus constitutes a powerful, versatile, and cost-effective nonmammalian model with which to investigate important contemporary issues of immunity relevant to human health such as ontogeny of immunity, self-tolerance, wound healing, autoimmunity, cancer immunity, immunotoxicology, and adaptation of host immune defenses to emerging pathogens. This model system presents several attractive features: an external developmental environment free of maternal influence that allows for easy experimental access from early life stages; an immune system that is remarkably similar to that of mammals; the availability of large-scale genetic and genomic resources; invaluable major histocompatibility complex (MHC)-defined inbred strains of frogs; and useful tools such as lymphoid tumor cell lines, monoclonal antibodies, and MHC tetramers. Modern reverse genetic loss-of-function and genome-editing technologies applied to immune function further empower this model. Finally, the evolutionary distance between Xenopus and mammals permits distinguishing species-specific adaptation from more conserved features of the immune system. In this introduction, the advantages and features of Xenopus for immunological research are outlined, as are existing tools, resources, and methods for using this model system.