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An efficient and eco-friendly workflow for dual fingermark processing and STR profiling.

Yinon Harush-Brosh1, Lina Mayuoni-Kirshenbaum2, Yakov Mashiach2

  • 1Institute of Dental Sciences, The Hebrew University - Hadassah School of Dental Medicine, POB 12272, Jerusalem, 9112102, Israel.

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Summary
This summary is machine-generated.

This study introduces a new, non-destructive method for collecting touch DNA and fingermarks (FM) simultaneously. The novel workflow enhances forensic identification by improving DNA recovery and fingermark visualization on various surfaces.

Keywords:
BVDA gel-lifterFingermarksForensic scienceShort tandem repeatsTouch DNAWet powder

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Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Touch DNA and fingermark (FM) analysis are crucial in criminal casework.
  • Current methods for FM processing can hinder subsequent DNA analysis.
  • A novel, non-destructive workflow using gel-lifters and eco-friendly visualization was developed.

Purpose of the Study:

  • To evaluate a new protocol for non-destructive fingermark collection and DNA recovery.
  • To assess the protocol's effectiveness across different substrates, time delays, and donor genders.
  • To determine the limitations and scope of the proposed workflow for forensic applications.

Main Methods:

  • Developed 120 fingermarks from 20 donors (10 male, 10 female).
  • Employed white BVDA gel-lifters and black Wetwop® solution for non-destructive FM collection and visualization.
  • Recovered and analyzed DNA from both the gel-lifter adhesive and acetate imprint for each FM.
  • Quantified DNA and performed genotyping, focusing on Short Tandem Repeat (STR) loci.

Main Results:

  • The protocol demonstrated higher efficiency on non-porous substrates (e.g., 65% on car tin).
  • Forensically useful DNA profiles were obtained from porous substrates like drywall (25% male, 15% female).
  • Dual analysis of DNA from the gel-lifter and acetate imprint increased donor identification and confidence.

Conclusions:

  • The novel workflow enables simultaneous, non-destructive collection of fingermarks and touch DNA.
  • This integrated approach significantly enhances personal identification rates and strengthens forensic evidence.
  • The protocol shows promise for improving casework efficiency in forensic investigations.