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Published on: November 26, 2014
Redox State Control of Human Cytoglobin by Direct Electrochemical Method to Investigate Its Function in Molecular
Yasuhiro Mie1, Kyoka Takahashi1, Ryo Torii2
1Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).
Abstract:
The direct electron transfer between human cytoglobin (Cygb) and the electrode surface, which would allow manipulating the oxidation states of the heme iron in Cygb, was first observed by immobilizing Cygb on a nanoporous gold (NPG) electrode via a carboxy-terminated alkanethiol. The voltammetric performances of the wild type and mutated Cygb-immobilized NPG electrodes were evaluated in the absence or presence of potential substrates. The obtained results demonstrated that the usefulness of the proposed method in understanding the function of Cygb in molecular basis.
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