Rhizosphere microbiome: Engineering bacterial competitiveness for enhancing crop production.

Area of Science:

• Agricultural Science
• Microbiology
• Plant Science

Background:

• Plants face significant abiotic and biotic stresses limiting growth and yield.
• Sustainable agriculture requires reduced chemical fertilization and agrochemicals.
• Plant microbiome manipulation is a promising strategy to enhance crop resilience.

Purpose of the Study:

• To review strategies for enhancing crop health and yield through microbiome engineering.
• To explore the potential of transferring beneficial rhizosphere microbiomes.
• To discuss gene editing techniques for improving plant stress resistance.

Main Methods:

• Review of current research on plant-microbiome interactions.
• Analysis of next-generation sequencing (NGS) and metagenomics for microbiome characterization.
• Examination of CRISPR/Cas9 gene editing for manipulating plant susceptibility genes.

Main Results:

• Rhizosphere microbiome engineering can improve nutrient acquisition and stress resistance.
• Transferring beneficial microbial communities from resistant to susceptible plants is feasible.
• CRISPR/Cas9 offers targeted strategies to enhance plant health by modifying susceptibility genes.

Conclusions:

• Microbiome manipulation and gene editing are powerful tools for sustainable agriculture.
• Understanding holobiont and microbiome interactions is key to developing resilient crops.
• This review provides foundational knowledge for optimizing plant health strategies.