Related Experiment Video
Updated: Dec 20, 2025

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Published on: February 21, 2019
Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids
Bankala Krishnarjuna1, Thirupathi Ravula, Ayyalusamy Ramamoorthy
1Biophysics Program, Department of Chemistry, Macromolecular Science and Engineering, University of Michigan, Arbor, MI 48109-1055, USA. ramamoor@umich.edu.
Abstract:
Despite their denaturing properties, detergents are used to purify and study membrane proteins. Herein, we demonstrated a polymer-based detergent-free extraction of the membrane protein cytochrome-b5 along with E. coli lipids. Nuclear magnetic resonance experiments revealed the suitability of using nanodiscs for high-resolution studies and revealed the types of native lipids associated with the protein.

