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Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue
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pCREB expression in human tissues from epilepsy surgery.

Dalia De Santis1, Laura Rossini1, Laura Tassi2

  • 1Epilepsy Unit, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy.

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|May 29, 2020
PubMed
Summary
This summary is machine-generated.

Phospho-CREB (pCREB) expression in brain tissue does not reliably mark epileptic foci. However, its layer-specific patterns may aid in studying malformations of cortical development, offering insights into brain development disorders.

Keywords:
SEEG recordingepilepsy surgeryhuman tissuepCREB expression

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pathology

Background:

  • Activity-dependent changes in epilepsy can serve as biomarkers for seizure propensity.
  • cAMP-response element binding protein (CREB) activation has been linked to interictal spikes in human epileptic foci.

Purpose of the Study:

  • To investigate phospho-CREB (pCREB) expression in drug-resistant focal epilepsy surgical samples.
  • To correlate pCREB expression with intracranial stereo-electroencephalography (SEEG) findings.
  • To assess pCREB as a potential biomarker for epileptic activity.

Main Methods:

  • Immunohistochemistry was used to analyze pCREB expression in neocortical and hippocampal specimens from epileptic and non-epileptic patients.
  • Samples included cryptogenic epilepsy, focal cortical dysplasia (FCD) type II, and hippocampi with/without sclerosis.
  • Control tissues and rat brains were used to validate antigenicity.

Main Results:

  • pCREB showed consistent layer II neuronal nuclear expression in normal cortical lamination, irrespective of epilepsy or interictal spikes.
  • In type II FCD and other developmental malformations, pCREB was scattered without laminar specificity.
  • Increased pCREB immunoreactivity was observed in type IIB FCD lesions, primarily in glial and balloon cells, not significantly different between epileptic and non-epileptic tissues overall.

Conclusions:

  • pCREB immunohistochemistry is not a reliable marker for identifying epileptic foci.
  • pCREB's layer-related expression suggests potential utility in studying malformations of cortical development.
  • Further research into pCREB's role in developmental brain disorders is warranted.