Novel fluorescent probe for the ratiometric detection of β-galactosidase and its application in fruit
Yanan Li1, Ning Duan1, Xiaoming Wu1
1Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing 100048, PR China.
Abstract:
A ratiometric fluorescent probe (Probe 1) was developed for the sensitive detection of β-galactosidase (β-gal) activity. Probe 1 detected β-gal activity in the range 0-1.0 U/mL, with a limit of detection of 0.025 U/mL. In addition, as different activities of β-gal added, the luminescent intensity of Probe 1 gradually increased, as observed under a 365 nm ultraviolet lamp. Moreover, this method is low-volume, 20 μL, and time-efficient, 45 min per measurement. Probe 1 was successfully used to measure the β-gal activity in real fruit samples in a qualitative manner, by the naked eye, fast semi-quantitative manner, by smartphone, or quantitative manner, by fluorescence spectrometer.
