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Updated: Dec 20, 2025

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

Fluorogenic probes for imaging cellular phosphatase activity.

Brandon S McCullough¹, Amy M Barrios¹

¹Department of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, UT 84112-0581, USA.

Current Opinion in Chemical Biology

|May 30, 2020

Summary

This summary is machine-generated.

Developing fluorescent probes allows visualization of protein phosphatase activity. Advances in probes enhance imaging capabilities and specificity, aiding biological understanding despite challenges in enzyme family selectivity.

Keywords:

Cellular imaging Fluorescent probe Protein phosphatase

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Area of Science:

Biochemistry
Molecular Biology
Cellular Imaging

Background:

Protein phosphorylation is crucial for cellular signaling.
Understanding protein phosphatase roles is limited.
Visualizing enzyme activity enhances biological insight.

Purpose of the Study:

To review progress in developing fluorescent probes for protein phosphatase activity visualization.
To highlight advancements in probe technology for biological imaging.

Main Methods:

Development of fluorescent probes with visible and near-infrared (near-IR) profiles.
Design of peptide-based probes for enhanced selectivity.

Main Results:

Probes with visible and near-IR properties improve tissue and whole-animal imaging.
Peptide-based probes show increased selectivity for target phosphatases.
Key challenges remain in achieving high selectivity within enzyme families.

Conclusions:

Fluorescent probes are advancing the study of protein phosphatase function.
Further validation is needed for widespread adoption of new probes.
Improved probes will enhance understanding of cellular signaling pathways.