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The Genome Structure of Ciprofloxacin-Resistant Mycoplasma Hominis Clinical Isolates

E A Kolesnikova1, N F Brusnigina1, M A Makhova1

  • 1Academician I.N. Blokhina Nizhny Novgorod Scientific Research Institute of Epidemiology and Microbiology, Federal Service for Surveillance on Customers Rights Protection and Human Wellbeing, Nizhniy Novgorod, 603950 Russia.

Acta Naturae
|June 2, 2020
PubMed

Insights

Ciprofloxacin resistance in Mycoplasma hominis is linked to specific gene mutations. Understanding these genetic changes in efflux systems and DNA gyrase is key to combating antimicrobial resistance.

Area of Science:

  • Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Mycoplasma hominis is a common human pathogen.
  • Ciprofloxacin resistance in M. hominis poses a clinical challenge.
  • Understanding the genetic basis of resistance is crucial for effective treatment.

Purpose of the Study:

  • To investigate the genome structure of ciprofloxacin-resistant Mycoplasma hominis clinical isolates.
  • To identify genetic mechanisms conferring resistance to ciprofloxacin.
  • To analyze the genetic homology and metabolic capabilities of these isolates.

Main Methods:

  • Whole-genome sequencing using the Illumina platform.
  • Analysis of protein sequence homology.
  • Identification and characterization of genes encoding efflux systems and resistance-conferring mutations.

Main Results:

  • High homology observed among the protein sequences of the studied M. hominis isolates.
  • Identification of genes for ABC transporters and MATE family proteins involved in efflux.
  • Specific mutations (Ser83Leu in DNA gyrase subunit A for M45/M57; Lys144Arg in topoisomerase IV subunit A for MH1866) linked to ciprofloxacin resistance.

Conclusions:

  • Ciprofloxacin resistance in M. hominis is primarily mediated by specific point mutations in DNA gyrase and topoisomerase genes.
  • Efflux systems likely play a role in the overall resistance profile.
  • The limited biosynthetic capabilities of M. hominis are associated with its catabolic gene predominance.
Keywords:
ABC transportersMATEMycoplasma hominisantibiotic resistance mechanismsgenome structuregyrA and parC genes

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