Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Osteoblasts during various functional states].

N V Rodionova

    Arkhiv Anatomii, Gistologii I Embriologii
    |December 1, 1988
    PubMed
    Summary

    Osteoblasts, the bone-building cells, exhibit diverse functional states during bone formation. These distinct morpho-functional types reflect varying stages of collagen synthesis and secretion.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The dynamics of proliferation and differentiation of osteogenic cells under supportive unloading.

    TSitologiia i genetika·2011
    Same author

    Interactions of cells in zones of bone resorption under microgravity and hypokinesia.

    Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology·2005
    Same author

    Mechanisms of gravity-dependent changes in the bone tissue.

    Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology·2004
    Same author

    Changes of cell-vascular complex in zones of adaptive remodeling of the bone tissue under microgravity conditions.

    Advances in space research : the official journal of the Committee on Space Research (COSPAR)·2004
    Same author

    Morpho-functional adaptations in the bone tissue under the space flight conditions.

    Journal of gravitational physiology : a journal of the International Society for Gravitational Physiology·2003
    Same author

    Ultrastructural changes in osteocytes in microgravity conditions.

    Advances in space research : the official journal of the Committee on Space Research (COSPAR)·2003

    Area of Science:

    • Bone biology
    • Cellular biology
    • Histology

    Background:

    • Osteoblasts are crucial for bone formation.
    • Understanding osteoblast heterogeneity is key to comprehending bone development and repair.
    • Previous studies suggested variations in osteoblast activity but lacked detailed functional classification.

    Purpose of the Study:

    • To investigate the heterogeneity of osteoblast populations during osteogenesis.
    • To define and characterize distinct morpho-functional states of osteoblasts.
    • To correlate these states with specific biosynthetic activities.

    Main Methods:

    • Electron microscopy of rat and rabbit femoral bone.
    • Cytochemical analysis.
    • Radioautography using radiolabeled precursors (3H-uridine, 3H-glycine, 35S-sulfate, 45Ca).

    Main Results:

    • Identified four distinct osteoblast morpho-functional states (Types I-IV).
    • Type I (young osteoblasts) and preosteoblasts showed high 35S-sulfate incorporation and alkaline phosphatase activity.
    • Type II (mature osteoblasts) were characterized by predominant collagenous protein biosynthesis.
    • Type III osteoblasts (secrete-depots) underwent destruction during secretion.
    • Type IV osteoblasts represented a poorly active state observed in declining osteogenesis areas.

    Conclusions:

    • Osteoblast populations are heteromorphic, displaying at least four distinct morpho-functional states.
    • These states are linked to specific biosynthetic processes, including collagen synthesis and sulfate incorporation.
    • The observed heterogeneity reflects the asynchronous nature of biosynthetic processes within osteoblasts during osteogenesis.
    • These findings highlight a specific functional characteristic of collagen-producing cells.

    Related Experiment Videos