Related Experiment Video
Updated: Dec 20, 2025
10:38
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
Published on: March 3, 2010
14.2K
Small molecule phosphorescent probes for O2 imaging in 3D tissue models.
Ruslan I Dmitriev1, Alina V Kondrashina, Klaus Koren
1Biochemistry Department, University College Cork, Cork, Ireland. d.papkovsky@ucc.ie.
Biomaterials Science
|June 3, 2020
Summary
New phosphorescent probes enable precise oxygen monitoring in 3D tissue models. These cell-penetrating probes offer improved bio-distribution and analytical performance for physiological experiments, enhancing oxygen sensing capabilities.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Analytical Chemistry
Background:
- Accurate monitoring of oxygenation is crucial for studying cell behavior in 3D tissue models.
- Existing phosphorescence-based oxygen probes lack optimal bio-distribution and analytical performance for these applications.
Purpose of the Study:
- To develop and evaluate novel cell-penetrating phosphorescent probes for oxygen imaging in 3D tissue models.
- To assess the bio-distribution, analytical performance, and cell viability effects of new probes.
Main Methods:
- Synthesis of cell-penetrating phosphorescent conjugates of a Pt(ii)-tetrakis(pentafluorophenyl)porphine (PtPFPP) dye via click-modification.
- Evaluation of hydrophilic glucose (Pt-Glc) and galactose (Pt-Gal) conjugates for oxygen sensing in various 3D cell models.
- High-resolution phosphorescence lifetime-based oxygen imaging (PLIM) was employed.
Main Results:
- Pt-Glc and Pt-Gal conjugates showed minimal aggregation and efficient in-depth staining at concentrations ≤10 μM.
- Pt-Glc probe demonstrated good analytical performance and minimal impact on cell viability in cancer spheroids, neural spheres, and brain tissue slices.
- Oligoarginine conjugates exhibited aggregation and unstable photophysical properties, rendering them unsuitable for oxygen sensing.
Conclusions:
- Hydrophilic PtPFPP conjugates, particularly Pt-Glc, are effective cell-penetrating probes for oxygen imaging in 3D tissue models.
- These novel probes offer improved performance over existing technologies for physiological experiments requiring oxygen monitoring.
Related Concept Videos
01:14Photoluminescence: Applications
913
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
913
03:31Labeling DNA Probes
9.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.1K