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Fabrication of viable cyborg cells with cyclodextrin functionality.

Baghali G Mathapa1, Vesselin N Paunov

  • 1Surfactant and Colloid Group, Department of Chemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UK. V.N.Paunov@hull.ac.uk.

Biomaterials Science
|June 3, 2020
PubMed
Summary
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We developed two methods to attach cyclodextrins (CDs) to yeast cells without harming them. These functionalized yeast cells can be used for drug delivery and environmental cleanup.

Area of Science:

  • Biotechnology
  • Materials Science
  • Chemical Engineering

Background:

  • Surface functionalization of biological materials is crucial for advanced applications.
  • Saccharomyces cerevisiae (yeast) cells offer a biocompatible platform for modification.
  • Cyclodextrins (CDs) are versatile molecules for complexation and surface modification.

Purpose of the Study:

  • To develop and evaluate methods for surface functionalization of yeast cells with cyclodextrins.
  • To quantify the extent of cyclodextrin grafting on yeast cell surfaces.
  • To assess the viability of functionalized yeast cells for potential applications.

Main Methods:

  • Covalent attachment of cyclodextrins using epichlorohydrin as a cross-linker.
  • Polyelectrolyte-mediated deposition of cyclodextrin sulfate onto cell surfaces.

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  • Quantification of grafted cyclodextrins via host-guest complexation with a dye.
  • Assessment of cell viability using fluorescein diacetate staining.

    • Main Results:

    • Two distinct, viable methods for yeast cell surface functionalization with cyclodextrins were established.
    • Grafting densities reached hundreds of millions of cyclodextrin molecules per cell, forming multiple layers.
    • Functionalized cells retained viability, confirmed by fluorescein diacetate assay.

    Conclusions:

    • Surface-functionalized yeast cells with cyclodextrins are viable and possess high grafting densities.
    • These modified cells hold significant potential for applications in drug delivery, biosensing, and environmental remediation.
    • The developed methods provide a robust platform for creating novel bio-interfaces for various technological uses.