Higher b-values improve the correlation between diffusion MRI and the cortical microarchitecture

In diffusion MRI (dMRI), it remains unclear to know how much increase of b-value is conveying additional biological meaning. We tested the correlations between cortical microarchitecture and diffusion metrics computed from standard (1000 s/mm²), high (3000 s/mm²), to very high (5000 s/mm²) b-value dMRI.

Healthy volunteers were scanned with a dMRI pulse sequence that was first optimized together with a T1-WI and T2-WI. Averaged cortical surface map of estimated myelin (T1-WI/T2-WI) was compared with surface maps of mean diffusivity (MD) computed from each b-value (MD₁₀₀₀, MD₃₀₀₀, and MD₅₀₀₀) and to surface map of mean kurtosis (MK computed from the 0-, 1000-, to 3000-s/mm² shells) in 360 cortical parcels using Spearman correlations, multiple linear regressions, and Akaike information criteria (AIC).

Surface map from MD₁₀₀₀ showed variations not related to myelin but the MD₃₀₀₀ and MD₅₀₀₀ maps inversely mirrored estimated myelin map; lower MD values being observed in more myelinated cortical areas. MK mirrored myelinated cortical areas. Quantitatively, Spearman correlations between myelin and MD became more and more negative as long as b-values increased while the correlation was positive between myelin and MK. Multiple regression models confirmed negative associations between myelin and MD that were significantly better from MD₁₀₀₀ to MD₃₀₀₀ and MD₅₀₀₀ (R² = 0.33, p < 0.001; R² = 0.43, p < 0.001; and R² = 0.50, p < 0.001) and positive association between myelin and MK (R² = 0.53, p < 0.001). Comparisons of the 3 statistical models showed the best performances with MK and MD₅₀₀₀ (AIC_MK < AIC_MD5000 < AIC_MD3000 < AIC_MD1000).

Higher b-values are more closely related to subtle cellular variations of the cortical microarchitecture.