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Fiber Optic Particle Plasmon Resonance-Based Immunoassay Using a Novel Multi-Microchannel Biochip
Chang-Yue Chiang1,2, Chien-Hsing Chen3, Chien-Tsung Wang4
1Graduate School of Engineering Science and Technology, National Yunlin University of Science and Technology, Yunlin 64002, Taiwan.
Sensors (Basel, Switzerland)
|June 4, 2020
Summary
This study introduces a compact, cost-effective fiber-optic particle plasmon resonance (FOPPR) sensor for simultaneous multi-sample detection. The novel system achieves high sensitivity and reproducibility for analyzing biological samples.
Area of Science:
- Biophotonics
- Biosensing Technology
- Optical Engineering
Background:
- Simultaneous detection of multiple samples is crucial for efficient biological analysis.
- Existing biosensor systems often face limitations in sensitivity, stability, and sample volume requirements.
- Fiber-optic particle plasmon resonance (FOPPR) offers a promising label-free detection method.
Purpose of the Study:
- To develop a novel multi-microchannel biochip FOPPR sensor system for simultaneous detection of multiple samples.
- To enhance system stability and sensitivity through integrated optical and mechanical design.
- To create a compact, cost-effective, and low-sample-volume biosensing platform.
Main Methods:
- Development of a multi-microchannel flow-cell biochip using plastic material for simultaneous analysis of five samples.
- Integration of a novel photoelectric system and a lock-in module to improve sensor performance.
- Utilizing calibration curves from real-time sensor response data after injecting known concentrations of sucrose, streptavidin, and anti-dinitrophenyl (anti-DNP) antibody.
Main Results:
- The multi-microchannel FOPPR sensor system demonstrated good reproducibility with a coefficient of variation (CV) below 10%.
- Achieved excellent refractive index resolution of 6.23 ± 0.10 × 10-6 refractive index unit (RIU).
- Established low detection limits for streptavidin (2.92 ± 0.28 × 10-8 g/mL) and anti-DNP antibody (7.48 ± 0.40 × 10-8 g/mL).
Conclusions:
- The developed multi-microchannel FOPPR biochip sensor system enables simultaneous detection of multiple samples with high sensitivity and stability.
- The compact and cost-effective design, requiring only 30 μL of sample per channel, makes it suitable for various applications.
- The system's performance in terms of reproducibility, resolution, and detection limits highlights its potential for advanced biological and chemical analysis.
