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Validation of a Method for the Determination of Total Chromium in Rat Feces by Inductively Coupled Plasma Optical
Keith E Levine1, Matthew D Stout2, Glenn T Ross1
1RTI International, Research Triangle Park, North Carolina, USA.
Abstract:
The validation of a method for the determination of total chromium in Fischer-344 rat feces by inductively coupled plasma optical emission spectrometry following a rapid, atmospheric-pressure microwave digestion is described. The performance of the method was evaluated over the concentration range of 5.00 to 200 microg Cr/g feces. Data for method linearity, accuracy, precision, digest stability, and storage stability are presented along with limit of detection and limit of quantitation data. Data from a cross-validation method for B6C3F1 mouse feces are also presented. Following validation, the method was applied to analyze samples collected in support of two chronic toxicological investigations.

