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Expression of lncRNA AK058003 in esophageal carcinoma and analysis of its intervention effect

P Zhang1, K Xiong, P Lv

  • 1Department of Cardiothoracic Surgery, Tianjin Medical University General Hospital, Tianjin, China. pengzhang01@tmu.edu.cn.

European Review for Medical and Pharmacological Sciences
|June 5, 2020
PubMed
Abstract

Insights

Long non-coding RNA (lncRNA) AK058003 is highly expressed in esophageal carcinoma (EC) tissues, promoting cancer cell proliferation and metastasis. High lncRNA AK058003 expression correlates with poorer patient prognosis, suggesting its potential as a diagnostic biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Esophageal carcinoma (EC) is a significant global health concern.
  • The role of long non-coding RNAs (lncRNAs) in cancer development is increasingly recognized.
  • Identifying novel biomarkers for EC diagnosis and prognosis is crucial.

Purpose of the Study:

  • To investigate the expression levels of lncRNA AK058003 in EC tissues.
  • To analyze the functional role of lncRNA AK058003 in EC cell proliferation, migration, and invasion.
  • To evaluate the potential of lncRNA AK058003 as a prognostic biomarker for EC.

Main Methods:

  • Quantitative Polymerase Chain Reaction (qPCR) to detect lncRNA AK058003 expression in patient tissues and cell lines.
  • Exogenous interference using lncRNA AK058003 siRNA in EC cell lines.
  • Cell proliferation assays (cell growth curve, cell cycle analysis).
  • Cell migration and invasion assays (wound healing assay).
  • Western blot analysis for matrix metalloproteinase (MMP) protein expression.
  • Correlation analysis between lncRNA AK058003 expression and clinical parameters (TNM stage).

Main Results:

  • lncRNA AK058003 was significantly upregulated in EC tissues compared to adjacent non-cancerous tissues.
  • Higher expression of lncRNA AK058003 was observed in EC cell lines versus normal esophageal epithelial cells.
  • Silencing lncRNA AK058003 with siRNA significantly inhibited EC cell proliferation, induced G0/G1 cell cycle arrest, and reduced cell migration and invasion.
  • Downregulation of MMP1 and MMP2 protein expression was observed after lncRNA AK058003 silencing.
  • High lncRNA AK058003 expression was associated with a worse postoperative prognosis in EC patients.

Conclusions:

  • lncRNA AK058003 is oncogenic in esophageal carcinoma, promoting tumor growth and metastasis.
  • lncRNA AK058003 plays a critical role in regulating cell cycle, proliferation, migration, and invasion.
  • lncRNA AK058003 serves as a potential prognostic biomarker for esophageal carcinoma.

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