Related Experiment Video
Updated: Dec 19, 2025
10:07
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
Published on: March 16, 2022
2.5K
Comment on "RNA-guided DNA insertion with CRISPR-associated transposases"
Phoebe A Rice1, Nancy L Craig2, Fred Dyda3
1Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, USA. price@uchicago.edu.
Science (New York, N.Y.)
|June 6, 2020
Summary
A CRISPR-Cas system offers programmable RNA-guided DNA insertions. However, the Tn7 transposon system may also insert the entire donor plasmid, not just the intended transposon.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR-Cas systems provide RNA-guided DNA targeting.
- Tn7-type transposons are mobile genetic elements used for DNA insertion.
Purpose of the Study:
- To evaluate a Tn7-type transposon-encoded CRISPR-Cas system for programmable DNA insertions.
- To identify potential limitations of the described system.
Main Methods:
- Utilized a Tn7-type transposon system.
- Employed CRISPR-Cas machinery for RNA-guided targeting.
- Analyzed insertion products.
Main Results:
- The system enables RNA-guided, programmable insertions.
- Biochemical properties of Tn7 suggest co-insertion of the donor plasmid.
Conclusions:
- The described CRISPR-Cas system shows promise for targeted DNA integration.
- Further investigation is needed to confirm the extent of plasmid co-insertion.
Related Concept Videos
01:28CRISPR/Cas9 Genome Editing
1.4K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.4K
01:59CRISPR
57.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.1K
02:53Conservative Site-specific Recombination and Phase Variation
6.5K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.5K
02:31Homologous Recombination
61.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
61.9K
02:53CRISPR and crRNAs
18.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.5K
02:57DNA-only Transposons
17.0K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
17.0K