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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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Compact off-axis holographic slide microscope: design guidelines.

Teresa Cacace1,2, Vittorio Bianco1, Biagio Mandracchia1

  • 1Institute of Applied Sciences and Intelligent Systems "E. Caianiello", Italian National Research Council (ISASI-CNR), Via Campi Flegrei 34, 80078, Pozzuoli (Napoli), Italy.

Biomedical Optics Express
|June 6, 2020
PubMed
Summary
This summary is machine-generated.

We developed a cost-effective, portable 3D-printed holographic microscope for field diagnostics. This label-free imaging tool analyzes diverse samples like microalgae and bacteria outside the lab.

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Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
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Area of Science:

  • Optics and Photonics
  • Microscopy
  • Biotechnology

Background:

  • Holographic microscopy offers label-free, high-throughput imaging for diagnostics and environmental monitoring.
  • Existing in-line holographic microscopes are often costly and limited to sparse samples.
  • Off-axis configurations can simplify phase retrieval but require careful design.

Purpose of the Study:

  • To present a 3D-printed, cost-effective, and field-portable off-axis holographic microscope.
  • To demonstrate a simplified, non-iterative phase retrieval method using hologram demodulation.
  • To provide design guidelines for developing modular and adaptable holographic microscopes.

Main Methods:

  • Construction of a 3D-printed, field-portable off-axis holographic microscope.
  • Utilized a holographic microfluidic slide for sample containment and imaging.
  • Employed hologram demodulation for non-iterative phase retrieval.
  • Tested the prototype with marine microalgae, polystyrene beads, E. coli bacteria, and microplastics.

Main Results:

  • Successfully imaged diverse microscopic samples including biological organisms and microplastics.
  • Achieved label-free, quantitative imaging with a simplified reconstruction process.
  • Demonstrated the potential for modularity and customization of the optical design.
  • Detailed performance and cost trade-offs for various design choices.

Conclusions:

  • The developed holographic microscope is a versatile, cost-effective tool for in situ analysis.
  • The design facilitates use by non-expert users for point-of-care testing and environmental monitoring.
  • This approach removes complexity from phase retrieval, making holographic microscopy more accessible.