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Updated: Dec 19, 2025

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Quantitative analysis of homocysteine in liquid by terahertz spectroscopy

Liping Wang1, Xu Wu1, Yan Peng1,2,3

  • 1Terahertz Technology Innovation Research Institute, Shanghai Key Lab of Modern Optical System, Terahertz Science Cooperative Innovation Center, University of Shanghai for Science and Technology, Shanghai Institute of Intelligent Science and Technology, Shanghai, China.

Biomedical Optics Express
|June 6, 2020
PubMed

Insights

This study introduces terahertz spectroscopy for rapid, accurate homocysteine detection in biological samples. The novel method overcomes limitations of conventional techniques, enabling real-time quantitative analysis for disease assessment.

Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Spectroscopy

Background:

  • Homocysteine is linked to cardiovascular disease and dementia.
  • Current detection methods (HPLC, FPIA) are slow and complex.
  • A need exists for faster, more accurate homocysteine quantification.

Purpose of the Study:

  • To develop a novel quantitative detection method for homocysteine.
  • To utilize terahertz (THz) spectroscopy for biological sample analysis.
  • To establish a rapid and accurate diagnostic tool for homocysteine levels.

Main Methods:

  • Quantitative detection of homocysteine using terahertz spectroscopy.
  • Development of a low-temperature sample drying pretreatment method.
  • Analysis of the linear relationship between THz spectral intensity and homocysteine concentration.

Main Results:

  • Achieved a detection limit of 10 µmol/L for homocysteine.
  • Demonstrated quantitative, accurate, and real-time detection capabilities.
  • THz spectroscopy showed a significantly higher correlation coefficient (0.99809) compared to Raman spectroscopy (0.80022).

Conclusions:

  • Terahertz spectroscopy offers a superior alternative for homocysteine detection.
  • The developed method is rapid, accurate, and suitable for real-time analysis.
  • This technique aids in the precise evaluation of pathological stages related to homocysteine levels.

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