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Fluorophosphonate-Based Degrader Identifies Degradable Serine Hydrolases by Quantitative Proteomics
S Denise Field1, Wankyu Lee1, Jason K Dutra2
1Pfizer Worldwide Research and Development, 1 Portland St, Cambridge, MA, 02139, USA.
Chembiochem : a European Journal of Chemical Biology
|June 6, 2020
Abstract:
Novel chemical biology probes linking a serine hydrolase-directed fluorophosphonate warhead and cereblon-binding pomalidomide were assessed for the degradation of serine hydrolases. A quantitative proteomics approach to detect degraded proteins revealed that, despite the engagement of ∼40 serine hydrolases, degradation was achieved for only a single serine hydrolase, lysophospholipase II (LYPLA2).

