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Microwave-assisted tissue processing, fixation and staining in tissues of different thicknesses: A comparative study
Mahesh Rao1, Shaila M Pai2, Sanjeev Balappa Khanagar3
1Department of Oral Pathology and Microbiology, RVS Dental College and Hospital, Coimbatore, Tamil Nadu, India.
Aim And Objectives:
The study aimed at assessment of microwave assisted tissue fixation, processing and staining and to determine if it can replace standard formalin fixed paraffin embedded processing in tissues of different thickness.
Materials And Methods:
Specimens from buccal mucosa and gingiva was used in the study and were divided into three different thickness and was fixed, processed and stained according to conventional method and with a use of kitchen microwave oven respectively. The present study is the first of its kind where oral tissues was fixed, processed and stained with a kitchen microwave in three different thickness. The results obtained was statistically analyzed using IBM SPSS Statistics version 21.0 software.
Results:
The new technique of fixation, tissue processing and staining using a microwave employed in the present study represented a major change from conventional method and achieved significant reduction in time taken.
Conclusion:
The ease of application and speed of this technique significantly reduced turnaround time in diagnostic labs.
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