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Updated: Dec 19, 2025
Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
Published on: October 7, 2021
Serum-Based Proteomics Reveals Lipid Metabolic and Immunoregulatory Dysregulation in Cervical Artery Dissection With
Yongtao Yang1, Jing Peng2, Suxia Wang3
1Department of Neurology, Chongqing Renji Hospital, University of Chinese Academy of Sciences, Chongqing, China.
Insights
Cervical artery dissection (CAD) is a major cause of stroke. Proteomic analysis identified six upregulated serum proteins, suggesting early immune and lipid metabolism changes in CAD pathophysiology and potential biomarkers for stroke risk.
Area of Science:
- Proteomics
- Biochemistry
- Neurology
Background:
- Cervical artery dissection (CAD) is a significant cause of stroke in younger populations.
- The underlying pathophysiological mechanisms of CAD are not well understood.
- Identifying biomarkers for CAD is crucial for early detection and risk assessment.
Purpose of the Study:
- To identify differentially expressed proteins in serum from patients with spontaneous cervical artery dissection (CAD) compared to non-CAD ischemic stroke.
- To investigate the pathophysiological pathways involved in CAD using proteomic analysis.
- To validate potential protein biomarkers for CAD detection.
Main Methods:
- Quantitative proteomic analysis using isobaric tagging for relative and absolute quantitation (iTRAQ) on serum samples.
- Bioinformatic analysis of differentially expressed proteins, including Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway overrepresentation.
- Enzyme-linked immunosorbent assay (ELISA) validation of six selected differential proteins.
Main Results:
- Proteomic analysis revealed significant differences in protein expression between CAD and non-CAD ischemic stroke subjects.
- KEGG pathway analysis indicated involvement of immunoregulation, blood coagulation, and lipid metabolism.
- Six proteins—apolipoprotein B, apolipoprotein C-I, lipopolysaccharide-binding protein, vascular cell adhesion molecule 1, fibulin-1, and ficolin-2—were significantly upregulated in CAD.
Conclusions:
- Proteomic analysis suggests early alterations in immunoregulation and lipid metabolism pathways are implicated in CAD pathophysiology.
- The identified panel of six proteins shows promise as serum-based biomarkers for detecting increased CAD risk in stroke patients.
- Further research is warranted to confirm the diagnostic and prognostic value of these protein biomarkers.
Abstract:
Cervical artery dissection (CAD) is an important causal factor for stroke in young and middle-aged individuals and presents a great burden to the individual stroke victim. However, the pathophysiological mechanisms underlying CAD remain unknown. Here, an iTRAQ (isobaric tagging for relative and absolute quantitation)-based quantitative proteomic approach was performed, to identify differentially expressed proteins in serum samples obtained from spontaneous CAD and non-CAD ischemic stroke subjects. Differential protein expression was analyzed for Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway overrepresentation, and six differential proteins were selected for enzyme-linked immunosorbent assay validation. Through KEGG analysis, the significantly differentiated proteins were primarily involved in immunoregulation, blood coagulation, and lipid metabolism. For the first time, differential expressions of apolipoprotein B, apolipoprotein C-I, lipopolysaccharide-binding protein, vascular cell adhesion molecule 1, fibulin-1, and ficolin-2 were confirmed as being significantly upregulated in CAD as compared to non-CAD ischemic stroke subjects. In conclusion, proteomic analysis reveals that early perturbation of immunoregulation and lipid metabolism may be involved in the pathophysiology of CAD. Specifically, the panel of six proteins identified is promising as serum-based biomarkers for the detection of increased CAD risk in stroke subjects.
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