Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Orchiectomy in horses: closed technique and primary closure of the incision.

Journal of the American Veterinary Medical Association·2026
Same author

Nature's blueprint: Exopolysaccharides linking microbiome dynamics to advanced bone tissue engineering.

Carbohydrate polymers·2026
Same author

A Single-Antenna RFID Machine Learning Approach for Direction and Orientation Tracking in Industrial Logistics.

Sensors (Basel, Switzerland)·2026
Same author

Feasibility of a hand-held myotonometry device for measuring biomechanical muscle parameters in horses.

American journal of veterinary research·2026
Same author

Shaping Function: Polymeric 3D Systems With Unconventional Geometries for Biomedical Applications.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Cell-in-Shell Metacells in Single-Cell Nanoencapsulation.

ChemPlusChem·2026

Related Experiment Video

Updated: Dec 19, 2025

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation
11:26

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation

Published on: June 17, 2014

16.9K

Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell

Hee Chul Moon1, Sol Han1, João Borges2

  • 1Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Korea. ischoi@kaist.ac.kr.

Soft Matter
|June 9, 2020
PubMed
Summary

Biodegradable nanofilms made from cationic starch and alginate can be controllably degraded using enzymes. This technology shows promise for biomedical applications like single-cell nanoencapsulation.

More Related Videos

Layer-by-layer Synthesis and Transfer of Freestanding Conjugated Microporous Polymer Nanomembranes
09:09

Layer-by-layer Synthesis and Transfer of Freestanding Conjugated Microporous Polymer Nanomembranes

Published on: December 15, 2015

9.7K
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

8.6K

Related Experiment Videos

Last Updated: Dec 19, 2025

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation
11:26

Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation

Published on: June 17, 2014

16.9K
Layer-by-layer Synthesis and Transfer of Freestanding Conjugated Microporous Polymer Nanomembranes
09:09

Layer-by-layer Synthesis and Transfer of Freestanding Conjugated Microporous Polymer Nanomembranes

Published on: December 15, 2015

9.7K
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

8.6K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Controlled build-up and degradation of cytocompatible nanofilms are crucial for nanomedicine.
  • Single-cell nanoencapsulation (SCNE) is a promising biomedical application requiring advanced materials.

Purpose of the Study:

  • To fabricate biodegradable cationic starch (c-ST) and alginate (ALG) nanofilms using layer-by-layer (LbL) assembly.
  • To investigate the controlled, enzyme-triggered degradation of these nanofilms.
  • To explore their application in SCNE and assess cytocompatibility.

Main Methods:

  • Fabrication of [c-ST/ALG] multilayer nanofilms via electrostatic LbL assembly.
  • Degradation studies using varying concentrations of α-amylase.
  • Surface characterization using quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry.
  • Assessment of DNA incorporation and release.
  • Evaluation of cytocompatibility during film formation and degradation on Saccharomyces cerevisiae.

Main Results:

  • Successfully fabricated biodegradable [c-ST/ALG] multilayer nanofilms.
  • Demonstrated on-demand, cytocompatible degradation of nanofilms using α-amylase.
  • Quantified degradation profiles under varying enzyme concentrations.
  • Showcased controlled DNA incorporation and release from the nanofilms.
  • Confirmed the cytocompatibility of the film-forming and degradation processes for S. cerevisiae.

Conclusions:

  • Cytocompatible, biodegradable nanofilms can be fabricated using c-ST and ALG via LbL assembly.
  • Enzymatic degradation with α-amylase provides a controlled method for nanofilm dissolution.
  • These nanofilms are suitable for SCNE applications, demonstrating high cytocompatibility.
  • The developed technology offers a pathway for advanced biomedical devices with programmed in vivo dissolution.