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Updated: Dec 19, 2025
An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
Published on: May 22, 2020
An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
Hannes Steinkellner1, Alexander V Beribisky2, Philip Mausberg2
1Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV); hannes.steinkellner@meduniwien.ac.at.
Abstract:
The ECLIA is a versatile method which is able to quantify endogenous and recombinant protein amounts in a 96-well format. To demonstrate ECLIA efficiency, this assay was used to analyze intrinsic levels of MeCP2 in mouse brain tissue and the uptake of TAT-MeCP2 in human dermal fibroblasts. The MeCP2-ECLIA produces highly accurate and reproducible measurements with low intra- and inter-assay error. In summary, we developed a quantitative method for the evaluation of MeCP2 protein variants that can be utilized in high-throughput screens.
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