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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
[Variability of Bacillus thuringiensis bacteriophages with C2-morphology]
Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia
|December 1, 1988
Summary
Two Bacillus thuringiensis bacteriophages, "17" and "7/13", share many properties but have a distinct deletion-insertion region, indicating they are closely related yet not identical.
Area of Science:
- Microbiology
- Virology
- Molecular Biology
Background:
- Bacillus thuringiensis bacteriophages are viruses that infect bacteria.
- Understanding bacteriophage properties is crucial for applications in biotechnology and pest control.
- Phage
- 17
- and
- 7/13
- are C2-morphology phages isolated from factory phagolysates.
Purpose of the Study:
- To investigate the biological and physicochemical properties of Bacillus thuringiensis bacteriophages
- 17
- and
- 7/13
- .
Main Methods:
- Comparative analysis of biological and physicochemical properties.
- Restriction endonuclease digestion (EcoRI, HindIII, SalGI, MvaI) for DNA mapping.
- Heteroduplex analysis to identify DNA sequence variations.
Main Results:
- Bacteriophages
- 17
- and
- 7/13
- exhibited identical lytic spectra, morphology, size, GC-content, and buoyant density.
- A physical map of the bacteriophage DNA was constructed using restriction endonucleases.
- Heteroduplex analysis revealed a 0.8 Md deletion-insertion loop, indicating a nonhomologous region.
Conclusions:
- Bacteriophages
- 17
- and
- 7/13
- are closely related due to shared characteristics.
- The identified deletion-insertion region confirms that these bacteriophages are not identical.
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