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Updated: Dec 19, 2025

Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide
Published on: May 12, 2023
Determination of free fatty acids in plasma by gas chromatography
Ken'ichi Ichihara1, Chihiro Kohsaka1, Naohiro Tomari1
1Kyoto Integrated Science & Technology Bio-Analysis Center, Kyoto Municipal Institute of Industrial Technology and Culture, 134 Cyudoji-Minamicho, Kyoto, 600-8813, Japan.
Abstract:
A method was developed for determination of free fatty acids (FFAs) in plasma by gas chromatography. Plasma was extracted with 3 vol of methanol. Most cholesterol esters and triacylglycerols did not dissolve in the aqueous methanol. FFAs in the crude lipid solution were directly and selectively methylated with (trimethylsilyl)diazomethane at room temperature. Fatty acid methyl esters (FAMEs) formed were extracted with hexane, and nonreactive phospholipids were washed out with 95% methanol. The partially purified FAME preparation was analyzed by gas chromatography. The composition and amount of plasma FFAs closely approximated those obtained using two different methods.
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